Recombinant Drosophila melanogaster Protein bottleneck (bnk)

Code CSB-YP328169DLU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP328169DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328169DLU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP328169DLU
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bnk
Uniprot No.
Alternative Names
bnk; CG1480; Protein bottleneck
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-303
Target Protein Sequence
MSISTFNFQF YNYKLSPSSP GFGSTASRSS SSCISELEMD IDEDMSAQPT ITSTPKPRFT SQLAVELAKT EPGNGISPLR PKLHTAQKRW SMELREKVLE MSKRNNGQEK PQTARQQEQR QPQEQPLQQE ELQHQQQEPT VTDKINFFNK LTNTFESGFS KLMPQASSTN NRFIAMLRTA RPQNVATTTA NSSTANSFLG SDHSLSGSVT GQVPPPKPKR LSATTAQFAT PHVPAMGVGK GGSQRKCSLR RKPSMDKSRA TISRQSSSAS VRTQNHAIME DLSLVVPVRL RIAEYEQRIS MSA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a regulator of the microfilament network governing cellularization of the embryo. Determines the timing of a key conformational transition in the cortical microfilament network: the proper coordination of membrane invagination and basal closure of the cells. To do this, bnk possibly physically links neighboring contractile units of the early cycle 14 microfilament network in a manner that prevents basal constriction until the proper stage has been reached. Bnk together with nullo and Sry-alpha may provide auxiliary functions, by acting both to stabilize a large and dynamic microfilament structure and regulate its functions.
Subcellular Location
Cytoplasm, cytoskeleton. Note=Colocalizes with the structural transitions in the microfilament network during cellularization. Association is observed only when the network assumes an aligned and tightly apposed hexagonal configuration.
Tissue Specificity
Restricted to the blastoderm.
Database Links

KEGG: dme:Dmel_CG1480

STRING: 7227.FBpp0085116

UniGene: Dm.1899

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