Recombinant Drosophila melanogaster Protein deadpan (dpn)

Code CSB-YP637696DLU
MSDS
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Source Yeast
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Code CSB-EP637696DLU
MSDS
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Source E.coli
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Code CSB-EP637696DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP637696DLU
MSDS
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Source Baculovirus
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Code CSB-MP637696DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dpn
Uniprot No.
Alternative Names
dpn; CG8704Protein deadpan
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-435
Target Protein Sequence
MDYKNDINSD DDFDCSNGYS DSYGSNGRMS NPNGLSKAEL RKTNKPIMEK RRRARINHCL NELKSLILEA MKKDPARHTK LEKADILEMT VKHLQSVQRQ QLNMAIQSDP SVVQKFKTGF VECAEEVNRY VSQMDGIDTG VRQRLSAHLN QCANSLEQIG SMSNFSNGYR GGLFPATAVT AAPTPLFPSL PQDLNNNSRT ESSAPAIQMG GLQLIPSRLP SGEFALIMPN TGSAAPPPGP FAWPGSAAGV AAGTASAALA SIANPTHLND YTQSFRMSAF SKPVNTSVPA NLPENLIHTL PGQTQLPVKN STSPPLSPIS SISSHCEESR AASPTVDVMS KHSFAGVFST PPPTSAETSF NTSGSLNLSA GSHDSSGCSR PLAHLQQQQV SSTSGIAKRD REAEAESSDC SLDEPSSKKF LAGAIEKSSS AWRPW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. In the larval brain, required to maintain the self-renewal and identity of type II neuroblasts by regulating the expression of the transcriptional repressor erm together with other self-renewal transcriptional repressors such as klu and E(spl)mgamma-HLH. As part of its role in neuroblasts development, has been shown to be a direct target of the Notch signaling pathway, however might work also independently of N/Notch. In the developing larval and pupal brain, required for mushroom body differentiation. Involved in sex determination and SXL transcription repression when in complex with the corepressor protein Groucho.
Gene References into Functions
  1. Dpn contributes to the establishment of Cut expression. PMID: 24086596
  2. Data suggest that Dpn plays important roles in maintaining NB self-renewal and specification of type II NBs in larval brains and that Dpn and Notch function independently in regulating type II NB proliferation and specification. PMID: 23056424
  3. Dpn is a partner and target of CK2, and raises the possibility that its repressor functions might also be regulated by phosphorylation. PMID: 16342413
  4. Groucho and associated maternal and zygotic bHLH repressors define the threshold X-linked signal element gene concentrations needed to activate Sex-lethal establishment promoter and they participate directly in sex signal amplification. PMID: 18773886
Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG8704

STRING: 7227.FBpp0087879

UniGene: Dm.525

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