Recombinant Escherichia coli Exodeoxyribonuclease V beta chain (recB), partial

1. Multiple ssDNA translocases within the RecBCD complex both before and after chi ensures processive unwinding of DNA substrates required for efficient recombination events. PMID: 29032606
2. This is the first occasion that RecBCD has been demonstrated to be inhibited by DNA adducts induced by cisplatin or UV. In addition, we quantified the amounts of DNA remaining after RecBCD treatment and observed that the level of inhibition was concentration and dose dependent. A DNA-targeted 9-aminoacridinecarboxamide cisplatin analogue was also found to inhibit RecBCD activity. PMID: 29129691
3. Extension of the 5'-tail of the unwound duplex induces a large conformational change in the RecD subunit, that is transferred through the RecC subunit to activate the nuclease domain of the RecB subunit. PMID: 27644322
4. A new role of the RecBCD complex in the processing of DNA single-strand gaps that are generated at DNA replication-blocking lesions: independently of its nuclease or helicase activities, the entire RecBCD complex is required for recombinational repair of the gap and efficient translesion synthesis. PMID: 28369478
5. Sequence-dependent nanometer-scale conformational dynamics of individual RecBCD-DNA complexes has been reported. PMID: 27220465
6. RecBCD is required to complete chromosomal replication. (Review) PMID: 26003632
7. A mutation within RecB (Y803H) that slows the primary translocation rate of RecBC also slows the secondary translocation rate to the same extent. PMID: 22820092
8. Study hypothesized that RuvAB catalyzes replication fork reversal, RecJ and XonA blunt the double-strand end, and then RecBCD loads RecA4142 onto this end to produce SOS(Con) expression. PMID: 20304994
9. crystal structure of RecBCD bound to a DNA substrate PMID: 15538360
10. RecBCD enzyme overproduction impairs DNA repair and homologous recombination in Escherichia coli. PMID: 15808933
11. Data suggest that RecBC and RecBCD destabilize six base pairs on binding to a blunt DNA duplex end in the absence of ATP, and that a loop can form on RecBC or RecBCD binding to DNA duplexes containing a pre-formed 3'-ssDNA tail with n > or =6 nucleotides. PMID: 16126227
12. Together, these results strongly indicate that RecD overproduction prevents dissociation of RecBCD enzyme from DNA substrate and thus increases its processivity. PMID: 16377056
13. The kinetics of the RecBCD-catalyzed reaction with small, single-stranded oligodeoxyribonucleotide substrates under single-turnover conditions using rapid-quench flow techniques, was studied. PMID: 16887145
14. Data suggest models for the molecular mechanism of Gam-mediated inhibition of RecBCD, and propose that Gam could be a mimetic of single-stranded, and perhaps also double-stranded, DNA. PMID: 17544443
15. Data show that Gam inhibits the binding of RecBCD to double-stranded DNA ends, even if RecBCD is bound to DNA before its interaction with Gam. PMID: 17583735
16. Data show that the RecBCD enzyme switches lead motor subunits in response to chi recognition. PMID: 18022364
17. genetic requirements for SOS induction after introduction of a double-strand break (DSB) by the I-SceI endonuclease in a RecA loading deficient recB mutant (recB1080) PMID: 18445487
18. Results describe the influence of DNA end structure on the mechanism of initiation of DNA unwinding by the Escherichia coli RecBCD and RecBC helicases. PMID: 18656489

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KEGG: ecj:JW2788

STRING: 316385.ECDH10B_2990