Code | CSB-EP600078HUb0 |
MSDS | |
Size | $224 |
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Is there any information on what procedures were used to isolate the protein and whether the protein was purified under native or denaturing conditions? If the latter, was the protein refolded?
After renaturation, the protein was purified by affinity chromatography. Ni column affinity chromatography is used for protein separation and purification. Taking EP system as an example, the general steps of purification are as follows: firstly, balance the column with buffer solution, and ultrasonically break the bacterial solution, then take the supernatant, filter and load the sample. secondly elute with eluents with different gradients, collect the corresponding elution peaks and detect the target bands.