Recombinant Human Alpha-2-macroglobulin receptor-associated protein (LRPAP1)

Code CSB-YP013104HU
MSDS
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Source Yeast
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Code CSB-EP013104HU
MSDS
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Source E.coli
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Code CSB-EP013104HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013104HU
MSDS
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Source Baculovirus
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Code CSB-MP013104HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
39 kDa receptor-associated protein; A2MRAP; A2RAP; Alpha 2 macroglobulin receptor associated protein; Alpha 2 MRAP; Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; AMRP_HUMAN; HBP44; Lipoprotein receptor associated protein; Low density lipoprotein receptor related protein associated protein 1; Low density lipoprotein receptor-related protein-associated protein 1; Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein; Low density lipoprotein related protein associated protein 1; low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1); Lrpap1; MGC138272; MRAP; MYP23; RAP
Species
Homo sapiens (Human)
Expression Region
35-357
Target Protein Sequence
YSREKN QPKPSPKRES GEEFRMEKLN QLWEKAQRLH LPPVRLAELH ADLKIQERDE LAWKKLKLDG LDEDGEKEAR LIRNLNVILA KYGLDGKKDA RQVTSNSLSG TQEDGLDDPR LEKLWHKAKT SGKFSGEELD KLWREFLHHK EKVHEYNVLL ETLSRTEEIH ENVISPSDLS DIKGSVLHSR HTELKEKLRS INQGLDRLRR VSHQGYSTEA EFEEPRVIDL WDLAQSANLT DKELEAFREE LKHFEAKIEK HNHYQKQLEI AHEKLRHAES VGDGERVSRS REKHALLEGR TKELGYTVKK HLQDLSGRIS RARHNEL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.
Gene References into Functions
  1. The clinical and biometric features we describe in this report characterize LRPAP1-related high myopia and should raise suspicion for mutations in the gene. PMID: 26271838
  2. The inhibition caused by reagents that prevented the activation of Rap was reversed by mobilizing intracellular calcium pharmacologically, whereas that caused by acrosome reaction inhibitors that impeded Rab3's binding to GTP was not. PMID: 25159528
  3. The LRPAP1 I allelic variant may be considered a candidate gene for PD, predominantly in patients having the APOE epsilon4 allelic variant. PMID: 24504617
  4. RAP inhibited light chain (LC)endocytosis by approximately 88% and ameliorated LC-induced cytokine responses and epithelial-to-mesenchymal transformation in human proximal tubular cells. PMID: 23894629
  5. Mutations in LRPAP1 are associated with severe myopia in humans. PMID: 23830514
  6. LRP1-C/T, A2M-Ile/Val and APOE-epsilon 2/epsilon 3/epsilon 4 polymorphisms are associated with AD. PMID: 20637261
  7. Findings reveal that RAP is a novel Abeta-binding protein that promotes cellular internalization of Abeta. PMID: 19826010
  8. Adenoviral-mediated expression of human receptor-asociated protein RAP in a murine model of von Willebrand disease results in a continuous rise in FVIII over 6 days. PMID: 11992244
  9. RAP is organized into three domains located in the first third (1D), middle third (2D), and last third (3D) of the protein; all three domains adopt stable tertiary structure as isolated domains and are monomers. PMID: 14674767
  10. NMR structure of domain 1 of receptor-associated protein PMID: 15213425
  11. An insertion allele is found to be associated with gallstone disease. PMID: 16704534
  12. RAP is expressed by thyrocytes in a TSH-dependent manner, both in cultured thyroid cells and in vivo PMID: 17123336
  13. analysis of the relationship between ACE and LRPAP1 gene polymorphisms and age in an Italian population PMID: 17536759
  14. Low-density lipoprotein receptor-related protein-associated protein (LRPAP1) gene IVS5 insertion/deletion polymorphism is not a risk factor for gallstone disease in a Polish population. PMID: 17913606
  15. Low-density lipoprotein receptor-related protein associated protein (LRPAP1) insertion/deletion polymorphism is associated with gallstone disease and gallbladder carcinoma PMID: 17987404
  16. LRP-1/LDLR-mediated uptake of A beta results in degeneration of perivascular cells. PMID: 18055545
  17. alterations of the RAP gene are not a common cause of hypothyroidism PMID: 18075286
  18. Our study suggests that LRPAP1-D and APOE E4 alleles significantly increase the susceptibility to degenerative dementias. PMID: 18721259

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Involvement in disease
Myopia 23, autosomal recessive (MYP23)
Subcellular Location
Rough endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment lumen. Golgi apparatus, cis-Golgi network. Golgi apparatus lumen. Endosome lumen. Cell surface.
Protein Families
Alpha-2-MRAP family
Database Links

HGNC: 6701

OMIM: 104225

KEGG: hsa:4043

STRING: 9606.ENSP00000421922

UniGene: Hs.40966

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