Product Details

Purity

>85% (SDS-PAGE)

Target Names

CHMP4C

Uniprot No.

Q96CF2

Alternative Names

Charged multivesicular body protein 4c; CHM4C_HUMAN; CHMP4c; Chromatin-modifying protein 4c; hSnf7-3; hVps32-3; SHAX3; SNF7 homolog associated with Alix 3; SNF7-3; Vacuolar protein sorting-associated protein 32-3; Vps32-3

Species

Homo sapiens (Human)

Expression Region

1-233

Target Protein Sequence

MSKLGKFFKG GGSSKSRAAP SPQEALVRLR ETEEMLGKKQ EYLENRIQRE IALAKKHGTQ NKRAALQALK RKKRFEKQLT QIDGTLSTIE FQREALENSH TNTEVLRNMG FAAKAMKSVH ENMDLNKIDD LMQEITEQQD IAQEISEAFS QRVGFGDDFD EDELMAELEE LEQEELNKKM TNIRLPNVPS SSLPAQPNRK PGMSSTARRS RAASSQRAEE EDDDIKQLAA WAT

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: upon phosphorylation by AURKB, together with ZFYVE19/ANCHR, retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ANCHR and VPS4 and subsequent abscission. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan.

Gene References into Functions

Data demonstrate the biological importance of the abscission checkpoint and suggest that dysregulation of abscission by CHMP4CT232 may synergize with oncogene-induced mitotic stress to promote genomic instability and tumorigenesis. PMID: 30181294
Results show that CHMP4C, under the regulation of the chromosomal passenger complex, binds to highly curved membranes and promotes the closure of membrane gaps. Two distinctly localized pools of phosphorylated CHMP4C exist during cytokinesisI but the phosphorylation is not required for its assembly into spiral filaments. Also, the centralspindlin complex associates preferentially with the with unphosphorylated form. PMID: 27784789
CHMP4C mediates radiation resistance in lung cancer cells. PMID: 26712741
The ESCRT-III subunit CHMP4B is a key effector in abscission, whereas its paralogue, CHMP4C, is a component in the abscission checkpoint that delays abscission until chromatin is cleared from the intercellular bridge. PMID: 26929449
AURKB phosphorylates CHMP4C at 3 serines its C-terminal tail.Phosphorylation at these sites appears essential for CHMP4C function because their mutation leads to cytokinesis defects. PMID: 22724069
Single nucleotide polymorphisms in CHMP4C gene is associated with ovarian cancer. PMID: 23535730
findings show CHMP4C is involved in abscission timing; this function correlated with its differential spatiotemporal distribution during late stages of cytokinesis; CHMP4C functioned in the Aurora B-dependent abscission checkpoint PMID: 22422861
The Bro1 domain of ALIX binds specifically to C-terminal residues of the human CHMP4C. PMID: 18511562

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Subcellular Location

Cytoplasm, cytosol. Late endosome membrane; Peripheral membrane protein. Midbody, Midbody ring.

Protein Families

SNF7 family

Tissue Specificity

Expressed in heart, spleen and kidney.

Database Links

HGNC: 30599

OMIM: 610899

KEGG: hsa:92421

STRING: 9606.ENSP00000297265

UniGene: Hs.183861

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Recombinant Human Charged multivesicular body protein 4c (CHMP4C)

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