Code | CSB-MP010509HU |
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Size | $440 |
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The production of recombinant human HLA class I histocompatibility antigen, alpha chain G (HLA-G) typically involves gene cloning, expression in a host system, and purification. The gene corresponding to the HLA-G protein (25-338aa) is cloned into a vector along with the N-terminal 6xHis-tag gene and then transformed into mammalian cells. The mammalian cells are cultured under conditions that promote protein expression. The expressed protein is then extracted and purified using methods like affinity chromatography, ensuring a high-quality product for various applications in related research. Its purity is greater than 90% as determined by SDS-PAGE.
HLA-G is a non-classical major histocompatibility complex (MHC) class I antigen with limited polymorphism, setting it apart from classical MHC-I antigens like HLA-A, HLA-B, and HLA-C [1][4]. HLA-G is uniquely expressed on extra-villous trophoblast cells at the fetal-maternal interface, where it protects the fetus from maternal uterine natural killer cell cytolysis [2][3].
HLA-G plays a crucial role in immune modulation by inhibiting the cytotoxicity of natural killer cells [2]. Research has shown that HLA-G upregulation is linked to cervical premalignant and malignant lesions [5]. This antigen is also significant for pregnancy maintenance, with studies indicating that a 14-bp deletion in the HLA-G gene may affect pregnancy outcomes [6][7].
References:
[1] X. Xu, Y. Zhou, & H. Wei, Roles of hla-g in the maternal-fetal immune microenvironment, Frontiers in Immunology, vol. 11, 2020. https://doi.org/10.3389/fimmu.2020.592010
[2] N. Rouas‐Freiss, R. Marchal, M. Kirszenbaum, J. Dausset, & E. Carosella, The α1domain of hla-g1 and hla-g2 inhibits cytotoxicity induced by natural killer cells: is hla-g the public ligand for natural killer cell inhibitory receptors?, Proceedings of the National Academy of Sciences, vol. 94, no. 10, p. 5249-5254, 1997. https://doi.org/10.1073/pnas.94.10.5249
[3] N. Rouas‐Freiss, R. Bras-Gonçalves, C. Menier, J. Dausset, & E. Carosella, Direct evidence to support the role of hla-g in protecting the fetus from maternal uterine natural killer cytolysis, Proceedings of the National Academy of Sciences, vol. 94, no. 21, p. 11520-11525, 1997. https://doi.org/10.1073/pnas.94.21.11520
[4] N. PATEL, M. Patel, P. Koringa, T. Shah, A. Patel, A. Tripathiet al., Human leukocyte antigen alleles, genotypes and haplotypes frequencies in renal transplant donors and recipients from west central india, Indian Journal of Human Genetics, vol. 19, no. 2, p. 219, 2013. https://doi.org/10.4103/0971-6866.116122
[5] N. Zheng, C. Wang, X. Zhang, D. Li, J. Zhang, S. Kanet al., Up‐regulation of hla‐g expression in cervical premalignant and malignant lesions, Tissue Antigens, vol. 77, no. 3, p. 218-224, 2011. https://doi.org/10.1111/j.1399-0039.2010.01607.x
[6] P. Tripathi, A. Abbas, S. Naik, & S. Agrawal, Role of 14‐bp deletion in the hla‐g gene in the maintenance of pregnancy, Tissue Antigens, vol. 64, no. 6, p. 706-710, 2004. https://doi.org/10.1111/j.1399-0039.2004.00308.x
[7] U. Shankarkumar, A. Shankarkumar, & K. Ghosh, Role of 14-bp deletion/insertion polymorphism in exon 8 of the hla-g gene in recurrent spontaneous abortion patients, Journal of Human Reproductive Sciences, vol. 4, no. 3, p. 143, 2011. https://doi.org/10.4103/0974-1208.92289
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I am interested in your product #CSB-MP010509HU, Recombinant Human HLA class I histocompatibility antigen, alpha chain G(HLA-G)
Could you let us know the gene introduction method to mammalian cell?
Thank you for sending the product. Unfortunately, on processing the stock we found the following discrepancy: * Product: CSB-MP010509HU (1.00mg) Recombinant Human HLA G protein (His tag) Lot number: 03853 Number of units ordered: 1 Number of units received: 1 x 1mg @ 0.15 mg/ml After centrifuging the product and then checking the content, we found that instead of the expected 6.67ml per vial, the vial received was short by 1ml. Could you please arrange for a free of charge replacement or top-up from the same lot number to compensate for this discrepancy? Many thanks in advance.