Recombinant Human Nidogen-1 (NID1), partial

Code CSB-YP015802HU
MSDS
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Source Yeast
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Code CSB-EP015802HU
MSDS
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Source E.coli
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Code CSB-EP015802HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015802HU
MSDS
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Source Baculovirus
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Code CSB-MP015802HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NID1
Uniprot No.
Alternative Names
Enactin; Ent; Entactin 1; Entactin; NID 1; NID; NID-1; Nid1; NID1_HUMAN; Nidogen (enactin); Nidogen 1; Nidogen; Nidogen-1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Gene References into Functions
  1. Results demonstrated that NID1 was a mesenchymal associated gene and its high expression was significantly correlated with shorter overall survival of ovarian cancer patients. The study presents evidence that NID1 promotes the migration, invasion and chemoresistance of ovarian cancer cells via EMT and its molecular mechanism involves the activation of ERK/MAPK signaling. PMID: 28416770
  2. Nidogen 1 (NID1) was confirmed to promote lung metastasis of breast cancer and melanoma. PMID: 28827399
  3. nidogen-1 degraded by CatS can be quantified in serum by the NIC assay. The current data strongly suggest that cathepsin-S degradation of nidogen-1 is strongly associated with NSCLC, which needs validation in larger clinical cohorts. PMID: 28282545
  4. Differential protein expression of collagen IV, laminin alpha2, and nidogen-1 indicated basal lamina remodeling develops in ischemic failing versus nonfailing human hearts. PMID: 26756417
  5. NID1 was confirmed as a target gene of MiR-192/215 in Hirschsprung's disease patients. PMID: 25857602
  6. Increase of NID1 protein expression is associated with endometrial cancer invasion. PMID: 25924802
  7. The cleavage of nidogen-1 and -2 is partially inhibited in human tumor samples. PMID: 23681936
  8. results demonstrate that mutation in either NID1 or LAMC1 disrupts the interaction NID1-LAMC1 complex; findings implicate the extracellular matrix in the pathogenesis of Dandy-Walker spectrum disorders PMID: 23674478
  9. Cleavage of nidogen-1 by cathepsin S impairs its binding to basement membrane partners PMID: 22952693
  10. NID1 gene is a biologically plausible locus for nevogenesis and melanoma development PMID: 21478494
  11. found to be ubiquitous component of basement membrane zones underneath developing epithelia of most of the major organ systems; may participate in cell biological functions during human development PMID: 12005023
  12. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone PMID: 17008882
  13. CpG islands of both NID1 and NID2 genes are aberrantly methylated in human cancer samples and cancer cell lines. PMID: 17328794

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Subcellular Location
Secreted, extracellular space, extracellular matrix, basement membrane.
Database Links

HGNC: 7821

OMIM: 131390

KEGG: hsa:4811

STRING: 9606.ENSP00000264187

UniGene: Hs.356624

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