Code | CSB-YP761526HDAM |
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In the general approach to express the recombinant human metapneumovirus (HMPV) matrix (M) protein, a plasmid encoding the human metapneumovirus matrix protein (1-254aa) and the N-terminal 6xHis-tag is first constructed. The constructed plasmid is then introduced into yeast cells. Yeast cells-containing plasmid are screened and cultured under conditions that induce the protein expression. Lysing the cultured cells and purifying the resulting recombinant matrix protein through affinity purification. The SDS-PAGE analysis is conducted to confirm the presence of the recombinant matrix protein and assess its purity. Its purity is over 85%.
HMPV is a significant pathogen associated with respiratory tract infections, particularly affecting infants and young children [1]. The genome of HMPV consists of various genes, including the matrix (M), fusion (F), and nucleoprotein (N) genes [2]. The matrix protein of HMPV plays a crucial role in the infectivity of the virus, as demonstrated in a study that quantitated its presence [3]. The matrix protein and other viral genes like the polymerase gene can detect the presence of HMPV due to their low variation rate [4].
References:
[1] J. Williams, P. Harris, S. Tollefson, L. Halburnt-Rush, J. Pingsterhaus, K. Edwardset al., "Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children", New England Journal of Medicine, vol. 350, no. 5, p. 443-450, 2004. https://doi.org/10.1056/nejmoa025472
[2] J. Greensill, P. McNamara, W. Dove, B. Flanagan, R. Smyth, & C. Hart, "Human metapneumovirus in severe respiratory syncytial virus bronchiolitis", Emerging Infectious Diseases, vol. 9, no. 3, p. 372-375, 2003. https://doi.org/10.3201/eid0903.020289
[3] S. Thammawat, T. Sadlon, P. Adamson, & D. Gordon, "Effect of sialidase fusion protein (das 181) on human metapneumovirus infection of hep-2 cells", Antiviral Chemistry and Chemotherapy, vol. 24, no. 5-6, p. 161-165, 2015. https://doi.org/10.1177/2040206616665971
[4] M. López-Huertas, I. Casas, B. Acosta-Herrera, M. García, M. Coiras, & P. Pérez-Breña, "Two rt-pcr based assays to detect human metapneumovirus in nasopharyngeal aspirates", Journal of Virological Methods, vol. 129, no. 1, p. 1-7, 2005. https://doi.org/10.1016/j.jviromet.2005.05.004
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