Recombinant Mouse Calcineurin subunit B type 1 (Ppp3r1)

Code CSB-YP737231MO
MSDS
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Source Yeast
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Code CSB-EP737231MO
MSDS
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Source E.coli
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Code CSB-EP737231MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP737231MO
MSDS
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Source Baculovirus
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Code CSB-MP737231MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Ppp3r1; CnbCalcineurin subunit B type 1; Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 3 regulatory subunit B alpha isoform 1
Species
Mus musculus (Mouse)
Expression Region
2-170
Target Protein Sequence
GNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Gene References into Functions
  1. Calcineurin B1 deficiency in glial cells impairs the small intestine and leads to malnutrition and eventual death in mice, suggesting that calcineurin plays a novel and important role in enteric glial cells. PMID: 29709916
  2. Expression of the RNA-binding protein, tristetraprolin (TTP) was down-regulated in response to calcineurin inhibition. PMID: 29723192
  3. Mice lacking Calcineurin B in CD11c(high)MHCII(+) cells (Cnb1 (CD11c) mice) spontaneously develop intestinal inflammation and are susceptible to induced colitis. PMID: 29549257
  4. data demonstrate that decreasing calcineurin activity in the amygdala increases anxiety-like behaviors and to some extent depression-like behaviors. PMID: 24742621
  5. necessary for the development of the smooth muscle wall of coronary arteries PMID: 23946498
  6. Macrophage Wnt-Calcineurin-Flt1 signaling regulates mouse wound angiogenesis and repair. PMID: 23303818
  7. intact calcineurin/NFAT signalling is critically required for p53 and senescence-associated mechanisms that protect against skin squamous cancer development PMID: 20485437
  8. Mechanistically, loss of Heart-specific CnB1 reduced expression of key Ca(2+)-handling genes that likely lead to arrhythmia and reduced contractility PMID: 20037164
  9. Calcineurin B1 is essential for positive selection during thymocyte development. PMID: 15030770
  10. gene expression of peroxisome proliferator-activated receptor gamma coactivator 1alpha and peroxisome proliferator-activated receptor alpha was reduced in the hearts of mice with cardiac-specific ablation of the Cn regulatory subunit. PMID: 15262994
  11. Endothelin 1 (ET-1) activates calcineurin and causes nuclear translocation of NFATc1, implicating the pathway in the ET-1-mediated stimulation of osteoblasts. PMID: 17237284
  12. Deletion substantially reduced skeletal muscle degeneration and histopathology compared with the sarcoglycan-null genotype alone. PMID: 17289669
  13. the inhibition of Cnb1 in osteoblasts increases bone mass by directly increasing osteoblast differentiation and indirectly decreasing osteoclastogenesis PMID: 17884821
  14. results indicate that early calcineurin/NFAT signalling produces a developmental period of ERK hypersensitivity, allowing very weak signals to induce positive selection PMID: 18046413
  15. gene deficiency results in reduced responsiveness of macrophages to LPS PMID: 19318421
  16. Report impaired contractile function and calcium handling in hearts of cardiac-specific calcineurin b1-deficient mice. PMID: 19700627

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Subcellular Location
Cytoplasm, cytosol. Cell membrane. Cell membrane, sarcolemma. Cell membrane; Lipid-anchor.
Protein Families
Calcineurin regulatory subunit family
Tissue Specificity
Brain specific.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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