Recombinant Mouse Collagen alpha-1 (XI) chain (Col11a1), partial

Code CSB-EP736811MO
MSDS
Size $472
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Target Names
Uniprot No.
Research Area
Others
Alternative Names
XI
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
1562-1804aa
Target Protein Sequence
DAGDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRISSWPKEKPGSWYSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSAAWYDVLSGSYDKALRFLGSNDEEMSYENNPHIKALYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQNQKFGFEVGPACFLG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
27.5 kDa
Protein Length
Partial
Tag Info
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Gene References into Functions
  1. Taken together, these results indicate that the transcription factor Sp1 upregulates the proximal promoter activity of the mouse Col11a1 gene in chondrocytes. PMID: 26487428
  2. these results indicate that the transcription factor NF-Y regulates the proximal promoter activity of mouse Col11a1 gene in chondrocytes PMID: 24092017
  3. Stickler syndrome and Marshall syndrome mutations in COL11A1 cause hearing loss via dominant negative effects upon wild-type fibrillar collagen polypeptides in the extracellular matrices of the cochlea. PMID: 12527136
  4. the cho mutation leads to osteoarthritis through a mechanism that does not initially involve mechanical factors. PMID: 13130470
  5. The expression patterns suggest essential roles for Col11a1 and Col11a2 in the basilar or tectorial membranes. PMID: 15141750
  6. Lef1 is an activator of the Col11a1 promoter and that Col11a1 suppresses terminal osteoblast differentiation PMID: 18280717

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Involvement in disease
Defects in Col11a1 are associated with chondrodysplasia, an autosomal recessive disease characterized by skeletal defects caused by abnormalities in the cartilage of limbs, ribs, mandibles and trachea.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Fibrillar collagen family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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