Recombinant Mouse DNA-directed RNA polymerase I subunit RPA34 (Cd3eap)

Code CSB-YP758837MO
MSDS
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Source Yeast
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Code CSB-EP758837MO
MSDS
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Source E.coli
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Code CSB-EP758837MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP758837MO
MSDS
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Source Baculovirus
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Code CSB-MP758837MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cd3eap
Uniprot No.
Alternative Names
Cd3eap; Ase1; Paf49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Anti-sense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3E-associated protein; RNA polymerase I-associated factor PAF49
Species
Mus musculus (Mouse)
Expression Region
1-399
Target Protein Sequence
MAGTQACSAT RFSCPPHFTE MSPDSEPSRF SLEALTGPDT ELWLIQAPAD FAPQCLNGRR VPLSGSKTVK GKLDGKKHRY RVFTSSPQAR EATLLASSSE AGGRLTCAPA PSGSLRIMEG PQEYLLSRVP LQLIPTSLPP QIPAGLRPRF SAFGGSPPVT GPGSASALRS PTSGKRKSTR KGTDASSDTQ EAVNRHGAME VKTALGNLGV SVKKRKRYFM QEEMEAKTME PVAELPVPSA TSSKKRKKSK GTETSQVEHT EPVAQTEPPE GTFLFPTKKR KRQKEADGTE EVDGIVADSQ PQVIVEAQEE TILLSPTKKR RKEKRQNLGM EAEMGPPGVL MVTEHSEHGL QAEVALVSPK KTKKKKGKRV GETEAALPDD FEPQGALAPS KKKERGARL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Involved in UBTF-activated transcription, presumably at a step following PIC formation.
Gene References into Functions
  1. yeast A34.5/A49 and mammalian PAF49/PAF53 heterodimeric complexes interact through species-specific sequence elements that appear to assume similar structural motifs. PMID: 22849406
  2. These results strongly suggest that RNA polymerase I (Pol I)-associated factor PAF49/ASE-1 plays an important role in rRNA transcription. PMID: 15226435
  3. Serial deletion analysis revealed that amino acids 199-338 were necessary for the nucleolar localization of PAF49. PMID: 18329376
Subcellular Location
Nucleus, nucleolus. Note=Accumulates in the nucleolus of exponentially growing cells.
Protein Families
Eukaryotic RPA34 RNA polymerase subunit family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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