Recombinant Mouse Glucosidase 2 subunit beta (Prkcsh)

Code CSB-YP018709MO
MSDS
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Source Yeast
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Code CSB-EP018709MO
MSDS
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Source E.coli
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Code CSB-EP018709MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018709MO
MSDS
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Source Baculovirus
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Code CSB-MP018709MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
PrkcshGlucosidase 2 subunit beta; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH
Species
Mus musculus (Mouse)
Expression Region
15-521
Target Protein Sequence
VEVKRP RGVSLSNHHF YEESKPFTCL DGTATIPFDQ VNDDYCDCKD GSDEPGTAAC PNGSFHCTNT GYKPLYILSS RVNDGVCDCC DGTDEYNSGT VCENTCREKG RKEKESLQQL AEVTREGFRL KKILIEEWKT AREEKQSKLL ELQAGKKSLE DQVETLRAAK EEAERPEKEA KDQHRKLWEE QQAAAKARRE QERAASAFQE LDDNMDGMVS LAELQTHPEL DTDGDGALSE EEAQALLSGD TQTDTTSFYD RVWAAIRDKY RSEVPPTDIP VPEETEPKEE KPPVLPPTEE EEEEEEEPEE EEEEEEEEEE APPPLQPPQP PSPTEDEKMP PYDEETQAII DAAQEARSKF EEVERSLKEM EESIRSLEQE ISFDFGPSGE FAYLYSQCYE LTTNEYVYRL CPFKLVSQKP KHGGSPTSLG TWGSWAGPDH DKFSAMKYEQ GTGCWQGPNR STTVRLLCGK ETVVTSTTEP SRCEYLMELM TPAACPEPPP EAPSDGDHDE L
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia.
Gene References into Functions
  1. TRIM67 regulates Ras signaling via degradation of 80K-H, leading to neural differentiation including neuritogenesis. PMID: 22337885
  2. Data demonstrate the presence of a binding site on the 3'-untranslated region of NR1 mRNA for AnxA2 and show the regulation of NR1 mRNA by AnxA2, GIIbeta and a third NR1 mRNA-binding protein, which is yet to be identified. PMID: 21995826
  3. 80K-H is a Ca(2+) sensor controlling TRPV5 channel activity PMID: 15100231
Subcellular Location
Endoplasmic reticulum.
Tissue Specificity
Expressed in kidney (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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