Recombinant Mouse Haptoglobin (Hp)

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Code CSB-EP010691MO
MSDS
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Cardiovascular
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
19-347aa
Target Protein Sequence
VELGNDAMDFEDDSCPKPPEIANGYVEHLVRYRCRQFYRLRAEGDGVYTLNDEKQWVNTVAGEKLPECEAVCGKPKHPVDQVQRIIGGSMDAKGSFPWQAKMISRHGLTTGATLISDQWLLTTAKNLFLNHSETASAKDITPTLTLYVGKNQLVEIEKVVLHPNHSVVDIGLIKLKQRVLVTERVMPICLPSKDYIAPGRVGYVSGWGRNANFRFTDRLKYVMLPVADQDKCVVHYENSTVPEKKNLTSPVGVQPILNEHTFCAGLTKYQEDTCYGDAGSAFAIHDMEEDTWYAAGILSFDKSCAVAEYGVYVRATDLKDWVQETMAKN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
40.9 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Gene References into Functions
  1. Both immunoblot analyses and enzymelinked immunosorbent assays confirmed the increased concentration of haptoglobin in crude mdx4cv serum. This suggests that haptoglobin, in conjunction with other altered serum proteins, represents a novel diagnostic, prognostic and/or therapymonitoring biomarker candidate to evaluate the inflammatory response in the mdx4cv animal model of dystrophinopathy. PMID: 28440464
  2. Data show that zonulin-dependent small intestinal barrier impairment is an early step leading to the break of tolerance with subsequent development of chronic inflammatory diseases (CIDs). PMID: 28423466
  3. the interaction between haptoglobin genotype and vitamin E affects atherosclerotic plaque progression and stability PMID: 25618031
  4. Suggest haptoglobin is an enhancer of inflammation after cardiac transplantation, contributing to graft rejection. PMID: 25801896
  5. Zonulin facilitates development of acute lung injury (ALI) both by enhancing albumin leak and complement activation as well as increased buildup of neutrophils and cytokines during development of ALI. PMID: 23564505
  6. Haptoglobin deficiency is associated with leakage-prone vessels via isolated VEGFA induction and/or suppressed Ang-1 expression, resulting in unbalanced VEGFalpha/Ang-1 transcription. PMID: 23274064
  7. Hp generated by the B-cell compartment, rather than by the liver, is functionally contributing to a normal immune response. PMID: 23548836
  8. the Hp2-2 protein is involved in aneurysm formation and that the Hp genotype may be a useful biomarker in predicting aneurysm progression PMID: 23096414
  9. Experimental models of colitis showed that haptoglobin has a protective role in inflammatory colitis PMID: 21708824
  10. these studies provide direct evidence that the increased oxidative stress in Hp 2-2 atherosclerotic plaques is due to hemoglobin derived iron. PMID: 19775690
  11. Synthetic PPAR gamma ligands selectively activate PPAR gamma bound to the haptoglobin gene promoter to arrest haptoglobin gene transcription. PMID: 19952271
  12. Hp may be regarded as a regulator of the Th1/Th2 balance in both human and murine immune systems. PMID: 11865978
  13. Hp plays a pivotal role in reducing renal oxidative damage during haemolysis. PMID: 11865980
  14. Haptoglobin gene expression regulation PMID: 11865981
  15. haptoglobin and hemopexin together are essential for protection from splenomegaly and liver fibrosis resulting from intravascular hemolysis PMID: 12393471
  16. the haptoglobin gene expression in adipocytes is stimulated by inflammatory cytokines, glucocorticoids, and the sympathetic system PMID: 14697247
  17. haptoglobin-mediated heme-iron recovery may contribute significantly to iron loading in hemochromatosis PMID: 15613548
  18. a direct linkage between diabetic vascular disease and the Hp genotype. PMID: 15852445
  19. Increased prevalence of diabetic retinopathy in individuals with the haptoglobin 2 genotype. PMID: 16742000
  20. Increased iron, lipid peroxidation and macrophage accumulation in ApoE-/- Hp 2-2 plaques suggests that the haptoglobin genotype plays a critical role in the oxidative and inflammatory response to intraplaque hemorrhage. PMID: 17068284
  21. The reduction in reverse cholesterol transport associated with diabetes mellitus was significantly greater in Hp2-2 mice as compared with Hp1-1 mice PMID: 17082477
  22. results suggest that correct processing of haptoglobin may be sensitive to ER stress & that, in absence of GRP78-mediated assistance, Hp is degraded; data show that iron regulates haptoglobin synthesis & suggest possible link with iron-induced ER stress PMID: 17644369
  23. Suggest that haptoglobin, by controlling plasma levels of hemoglobin, participates in the regulation of ferroportin expression, thus contributing to the regulation of iron transfer from duodenal mucosa to plasma. PMID: 17919498
  24. the Hp 2-2 genotype is critical for the development of severe vasospasm, which typically occurs 24 hours after SAH in mice PMID: 17962599
  25. The phenotype of Hp-deficient mice suggests a major regulatory activity for Hp in supporting proliferation and functional differentiation of B and T cells as part of homeostasis and in response to antigen stimulation. PMID: 18436583
  26. Pharmacogenomic effect of vitamin E on kidney structure and function in transgenic mice with the haptoglobin 2-2 genotype and diabetes mellitus. PMID: 19176700
  27. HDL can become proinflammatory via the hemoglobin x Hp pathway in mice and humans PMID: 19433579
  28. In diabetic mice the Hp 2-2 genotype is associated with increased mortality and more severe cardiac remodeling 30 days after myocardial infarct. PMID: 19490627
  29. Results suggest that HP plays a modulatory and protective role on autoimmune inflammation of the CNS. PMID: 19795414

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Subcellular Location
Secreted.
Protein Families
Peptidase S1 family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links
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