Recombinant Mouse Platelet-activating factor acetylhydrolase (Pla2g7)

Code CSB-YP736762MO
MSDS
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Source Yeast
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Code CSB-EP736762MO
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP736762MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pla2g7
Uniprot No.
Alternative Names
Pla2g7; Pafah; Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; EC 3.1.1.47; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; LDL-associated phospholipase A2; LDL-PLA(2); PAF 2-acylhydrolase
Species
Mus musculus (Mouse)
Expression Region
22-440
Target Protein Sequence
FHWQDTSSF DFRPSVMFHK LQSVMSAAGS GHSKIPKGNG SYPVGCTDLM FGYGNESVFV RLYYPAQDQG RLDTVWIPNK EYFLGLSIFL GTPSIVGNIL HLLYGSLTTP ASWNSPLRTG EKYPLIVFSH GLGAFRTIYS AIGIGLASNG FIVATVEHRD RSASATYFFE DQVAAKVENR SWLYLRKVKQ EESESVRKEQ VQQRAIECSR ALSAILDIEH GDPKENVLGS AFDMKQLKDA IDETKIALMG HSFGGATVLQ ALSEDQRFRC GVALDPWMYP VNEELYSRTL QPLLFINSAK FQTPKDIAKM KKFYQPDKER KMITIKGSVH QNFDDFTFVT GKIIGNKLTL KGEIDSRVAI DLTNKASMAF LQKHLGLQKD FDQWDPLVEG DDENLIPGSP FDAVTQVPAQ QHSPGSQTQN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent proinflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in lipoprotein particles and uncontrolled proinflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage.
Gene References into Functions
  1. Resveratrol downregulates Lp-PLA2 expression by inhibiting oxidative stress via SIRT1 pathway in a mouse model of chronic inflammation. PMID: 28608449
  2. Lp-PLA2 augments the inflammatory response after MI and antagonizes healing by disrupting the balance between inflammation and repair. PMID: 26232205
  3. Plg from mouse plasma contains oxPtdPC adducts that are not affected by the action of Lp-PLA(2), suggesting that linkage to Plg protects oxPtdPCs from metabolism during their transport in the plasma. PMID: 21614211
  4. SAA up-regulates Lp-PLA2 production significantly via a FPRL1/MAPKs./PPAR-gamma signaling pathway. PMID: 23623642
  5. Deletion of Pla2g7 decreases small intestinal polyp and colon tumor incidence in ApcMin/+ mice. PMID: 23361301
  6. Pla2g7 and Tnfrsf21 have been identified as genetic susceptibility to influenza genes in mice. PMID: 22427645
  7. Pla2g7 plays a crucial physiological role in smooth muscle cell differentiation from stem cells, and interactions between Nrf3 and Pla2g7 are essential. PMID: 22247257
  8. The effects of a specific lp-PLA2 inhibitor on atherosclerosis in ApoE-deficient mice and its associated mechanisms, are reported. PMID: 21909350
  9. Studies designed to evaluate the ability of precursor forms of PAF-AH to mature to fully active proteins indicated that the N-terminal end of human and mouse PAF-AH played important and opposite roles in this process. PMID: 20331434
  10. PAF acetylhydrolase activity in the uterus in early pregnancy was not produced locally but probably resulted from the influx of the plasma form of the enzyme PMID: 11833932
  11. deletion of both subunits induces a marked loss of germ cells at an early spermatogenic stage. PMID: 12551946
  12. PAF acetylhydrolases play key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo PMID: 16371369
  13. over-expression of the PAF-AH (I) catalytic subunits induces centrosomal amplification and microtubule disorganization by disturbing intracellular localization of LIS1 PMID: 17903175
  14. inactivation of PAF, produced by TEC, by the overexpression of plasma PAF-AH affects survival, migration, and the angiogenic response of TEC both in vitro and in vivo PMID: 17908960
  15. mouse and human PAF-AHs associate with human HDL particles of different density PMID: 18434304

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Subcellular Location
Secreted, extracellular space.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Plasma.
Database Links
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