Recombinant Mouse Sodium-dependent phosphate transport protein 2B (Slc34a2), partial

Code CSB-YP887584MO
MSDS
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Source Yeast
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Code CSB-EP887584MO
MSDS
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Source E.coli
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Code CSB-EP887584MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887584MO
MSDS
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Source Baculovirus
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Code CSB-MP887584MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Slc34a2; Npt2b; Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; Na(+)-dependent phosphate cotransporter 2B; Sodium/phosphate cotransporter 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; Solute carrier family 34 member 2
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in actively transporting phosphate into cells via Na(+) cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.
Gene References into Functions
  1. Npt2b deficiency did not affect Pi absorption within the range of Pi concentrations that normally occurs after meals. PMID: 29128884
  2. This study validated the role of Npt2b in the pathogenesis of Pulmonary alveolar microlithiasis (PAM). PMID: 26560359
  3. Overall, our preclinical results suggest that the ADC targeting NaPi2b provides an effective new therapy for the treatment of NSCLC and ovarian cancer and is currently undergoing clinical developments. PMID: 26156394
  4. NaPi-IIb is the only luminal Na(+) -dependent Pi transporter in the murine ileum and its absence is fully compensated for in adult females by a mechanism involving the bone-kidney axis. PMID: 25827490
  5. Knockdown of the sodium-dependent phosphate co-transporter 2b (NPT2b) suppresses lung tumorigenesis. PMID: 24194864
  6. B-RAF increases the cell surface protein abundance and activity of the type II Na+-coupled phosphate transporters NaPi-IIa and NaPi-IIb. PMID: 24258620
  7. that NHERF1 associates with NaPi-2b in enterocytes and regulates NaPi-2b adaptation. PMID: 22904329
  8. Compared with wild-type uremic mice, Npt2b-deficient uremic mice had significantly lower levels of serum phosphate and attenuation of FGF23. PMID: 22859851
  9. Npt2b is an important target for the prevention of hyperphosphatemia PMID: 21816756
  10. NaPi-IIb was the predominantly expressed epithelial isoform of the sodium-inorganic phosphate cotransporter and was markedly overexpressed in the proximal region in the infertile knockout compared to the fertile heterozygous c-ros transgenic mouse PMID: 12773415
  11. SGK1 stimulates the NaPi IIb, at least in part, by phosphorylating and thereby inhibiting Nedd4-2 binding to its target. PMID: 15044175
  12. along the entire mouse small intestine, low-phosphate diet affects transcription and/or the stability of NaPi-IIb mRNA PMID: 15701623
  13. NaPi-IIb expression was found to be mainly confined to the ileum in the small intestine PMID: 15701624
  14. In the steady state, NaPi-IIb showed a curvilinear I-V relationship, with rate-limiting behavior only for depolarizing potentials. PMID: 17342377
  15. Expression patterns of NaPi-IIb suggest that NaPi-IIb plays an important role in Pi absorption from maternal circulation. PMID: 19233126

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
SLC34A transporter family
Tissue Specificity
Highly abundant in the ileum of small intestine, whereas it is almost absent in the duodenum and in the jejunum.
Database Links
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