Product Details

Purity

>85% (SDS-PAGE)

Target Names

Usp2

Uniprot No.

O88623

Alternative Names

Usp2; Ubp41; MNCb-0190; Ubiquitin carboxyl-terminal hydrolase 2; EC 3.4.19.12; 41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2

Species

Mus musculus (Mouse)

Expression Region

1-613

Target Protein Sequence

MSQLSSTLKR YTESSRYTDA PYAKPGYGTY TPSSYGANLA ASFLEKEKLG FKPVSPTSFL PRPRTYGPSS ILDCDRGRPL LRSDIIGSSK RSESQTRGNE RPSGSGLNGG SGFSYGVSSN SLSYLPMNAR DQGVTLSQKK SNSQSDLARD FSSLRTSDGY RTSEGFRIDP GNLGRSPMLA RTRKELCALQ GLYQAASRSE YLTDYLENYG RKGSAPQVLT QAPPPSRVPE VLSPTYRPSG RYTLWEKSKG QASGPSRSSS PGRDTMNSKS AQGLAGLRNL GNTCFMNSIL QCLSNTRELR DYCLQRLYMR DLGHTSSAHT ALMEEFAKLI QTIWTSSPND VVSPSEFKTQ IQRYAPRFMG YNQQDAQEFL RFLLDGLHNE VNRVAARPKA SPETLDHLPD EEKGRQMWRK YLEREDSRIG DLFVGQLKSS LTCTDCGYCS TVFDPFWDLS LPIAKRGYPE VTLMDCMRLF TKEDILDGDE KPTCCRCRAR KRCIKKFSVQ RFPKILVLHL KRFSESRIRT SKLTTFVNFP LRDLDLREFA SENTNHAVYN LYAVSNHSGT TMGGHYTAYC RSPVTGEWHT FNDSSVTPMS SSQVRTSDAY LLFYELASPP SRM

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues. Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability. Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1.; Circadian clock output effector that regulates Ca(2+) absorption in the small intestine. Probably functions by regulating protein levels of the membrane scaffold protein PDZD3 in a rhythmic manner, and is therefore likely to control Ca(2+) membrane permeability mediated by the Ca(2+) channel TRPV6 in the intestine.

Gene References into Functions

3,3'-Diindolylmethane directly inhibits USP2 deubiquitinase activity in 3T3-L1 pre-adipocytes. PMID: 28586165
Usp2 is required for the PTH1-34-induced proliferation of osteoblasts PMID: 26643174
Usp2 is a clock output effector related to bodily Ca2+ homeostasis, a feature that is conserved across evolution. PMID: 26756164
A novel function of USP2 in the molecular clock in which it regulates PER1 function. PMID: 25238854
A role for USP2-45 as a central regulator of MR, able to remove the receptor monoubiquitylation and induced its destabilization. PMID: 23548632
Data suggest that USP2 does not play a primary role in the control of sodium balance or blood pressure. PMID: 23552861
a functional liver clock is required for the proper nutritional and circadian regulation of USP2-45 expression; at the molecular level, transcriptional coactivators PGC-1alpha and PGC-1beta and repressor E4BP4 exert opposing effects on USP2-45 promoter activity PMID: 23133608
USP2a potentially mediates circadian disruption by suppressing the CRY1 degradation during inflammation. PMID: 22669941
These studies delineate a novel pathway that links hormonal and circadian signals to gluconeogenesis and glucose homeostasis. PMID: 22447855
Rhythmic expression of USP2 in the SCN and other tissues offers a new level of control of the clock machinery through de-ubiqutinylation and suggests a role for USP2 during circadian adaptation to environmental day length changes PMID: 21966515
severe defect in the ability of Usp2 -/- spermatozoa to fertilize eggs PMID: 21543767

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Subcellular Location

Cytoplasm. Cytoplasm, perinuclear region.; [Isoform 2]: Nucleus. Membrane; Peripheral membrane protein. Cytoplasm.

Protein Families

Peptidase C19 family, USP2 subfamily

Tissue Specificity

Isoform 1: Expressed in heart, liver, kidney, pancreas and to a lower extent in skeletal muscle, brain and testis (at protein level). Expressed in testis, brain, heart and skeletal muscle. Not detected in the small intestine. Isoform 2: Expressed in the s

Database Links

KEGG: mmu:53376

STRING: 10090.ENSMUSP00000034508

UniGene: Mm.272770

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Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 2 (Usp2)

Product Details

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Target Background

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