Product Details

Purity

>85% (SDS-PAGE)

Target Names

Usp18

Uniprot No.

Q9WTV6

Alternative Names

Usp18; Ubp43Ubl carboxyl-terminal hydrolase 18; EC 3.4.19.-; 43 kDa ISG15-specific protease; ISG15-specific-processing protease; Ubl thioesterase 18

Species

Mus musculus (Mouse)

Expression Region

1-368

Target Protein Sequence

MGKGFGLLRK PCQSVVAEPQ QYSALEEERT MKRKRVLSRD LCSAWDSPHG LVGLHNIGQT CCLNSLLQVF MMNMDFRMIL KRITVPRSAE ERKRSVPFQL LLLLEKMQDS RQKAVLPTEL VQCLQKYNVP LFVQHDAAQL YLTIWNLTKD QITDTDLTER LQGLFTIWTQ ESLICVGCTA ESSRRSKLLT LSLPLFDKDA KPLKTLEDAL RCFVQPKELA SSDMCCESCG EKTPWKQVLK LTHLPQTLTI HLMRFSARNS RTEKICHSVN FPQSLDFSQV LPTEEDLGDT KEQSEIHYEL FAVIAHVGMA DFGHYCAYIR NPVDGKWFCF NDSHVCWVTW KDVQCTYGNH RYRWRETAYL LVYTKTGS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Involved in the negative regulation of the inflammatory response triggered by type I interferon. Upon recruitment by STAT2 to the type I IFN receptor subunit IFNAR2 interferes with the assembly of the ternary interferon-IFNAR1-IFNAR2 complex and acts as a negative regulator of the type I IFN signaling pathway. Also regulates protein ISGylation. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Gene References into Functions

Usp18 protects the cells from oxidative stress-induced apoptosis which may be through the regulation of p53 and caspase 3. PMID: 28557172
associates with and deubiquitinates TAK1 to protect against hepatic steatosis, insulin resistance, and the inflammatory response PMID: 28718215
this study identifies the osteopenia phenotype of mice lacking Usp18, which is known as a negative regulator of type I IFN signaling PMID: 27016605
USP18 has a protective role in pathological cardiac remodeling in mouse. PMID: 27572150
USP18's ISG15 specificity is mediated by a small interaction interface. PMID: 28165509
findings show that multiple inflammatory stimuli can modulate interferon stimulated gene expression and thus inhibit hepatocyte interferon signaling via USP18 induction PMID: 27009955
Increased ISGylation was accompanied by enhanced viral resistance without causing detrimental side effects suggesting that USP18 protease inhibition might be a suitable antiviral strategy PMID: 25805508
USP18 null mice develop leiomyosarcoma recapitulating key features of clinical leiomyosarcomas and patients with reduced-USP18 tumor levels have an unfavorable outcome. PMID: 26555296
USP18 lack in microglia causes destructive interferonopathy of the mouse brain. PMID: 25896511
results suggest that increasing ISGylation by specific inhibition of USP18 protease activity could constitute a promising antiviral strategy with only a minimal risk of severe adverse effects PMID: 25605921
USP18 is crucial for IFN-gamma-mediated inhibition of B16 melanoma tumorigenesis and antitumor immunity. PMID: 24884733
Usp18-driven enforced viral replication in dendritic cells can break immunological tolerance and critically influences induction of autoimmunity. PMID: 24204252
Hypersensitivity of Usp18 knockout in mammary epithelial cells to IFN-gamma enhances Cxcl10 expression and protects against mammary tumour progression. PMID: 23681607
Usp18 upregulation was associated with Wilms Tumor. PMID: 23291318
although UBP43 depletion can cause hypersensitivity to interferon-alpha/beta-mediated apoptosis in a broad range of cell types, the downstream pathway may vary depending on the cell type. PMID: 22683641
Usp18 modulates conventional CD11b+ dendritic cell development via its inhibitory effect on type I IFN signaling. PMID: 22491252
These results show the impact of USP18 in the development of Salmonella-induced typhlitis. PMID: 21614019
UBP43 (USP18) specifically removes ISG15 from conjugated proteins PMID: 11788588
UBP43 plays a critical role in maintaining the homeostatic balance of ISG15-conjugated protein, and that regulation of cellular levels of ISG15 protein modification is essential for brain cell function. PMID: 12208842
These findings identify UBP43 as a novel negative regulator of IFN signaling and suggest the involvement of protein ISGylation in the regulation of the JAK-STAT pathway. PMID: 12600939
UBP43 protein levels are regulated by proteolysis via the SCFSkp2 ubiquitin ligase PMID: 15342634
UBP43 (USP18), an ISG15 protease, and possibly protein ISGylation have a role in innate immunity against viral infection. PMID: 15531891
The phenotype of UBP43(-/-) mice was not rescued by the absence of ISG15, as evident from unchanged mortality, neurological symptoms, and occurrence of hydrocephalus. PMID: 16314524
Lack of UBP43, but not the increase of protein ISGylation, is related to the increased IFN signaling in Ubp43-deficient mice. PMID: 16382139
Results show that Ubp43 negatively regulates type I interferon signaling independent of its isopeptidase activity towards ISG15. PMID: 16710296
Deletion of Ubp43 causes an increase in free and conjugated Isg15 at the feto-maternal interface. PMID: 17381847
Steady-state level of hepatitis B virus (HBV)DNA is substantially reduced in UBP43-deficient mice in comparison to wild-type controls and suggests that HBV infection is more rapidly cleared if UBP43 expression is reduced. PMID: 18941237
Epstein-Barr virus independent dysregulation of UBP43 expression alters interferon-stimulated gene expression in Burkitt lymphoma PMID: 19551150
Results identify the inhibitor USP18/UBP43 as the key mediator of alpha-interferon-induced, long-lasting refractoriness of JAK-STAT signaling in the mouse liver. PMID: 19564419

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Protein Families

Peptidase C19 family

Database Links

KEGG: mmu:24110

STRING: 10090.ENSMUSP00000032198

UniGene: Mm.326911

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Recombinant Mouse Ubl carboxyl-terminal hydrolase 18 (Usp18)

Product Details

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Target Background

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