Recombinant Pig Orexin receptor type 1 (HCRTR1), partial

Code CSB-YP010231PI
MSDS
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Source Yeast
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Code CSB-EP010231PI
MSDS
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Source E.coli
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Code CSB-EP010231PI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010231PI
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP010231PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
HCRTR1; Orexin receptor type 1; Ox-1-R; Ox1-R; Ox1R; Hypocretin receptor type 1
Species
Sus scrofa (Pig)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.
Gene References into Functions
  1. 39 of 41 in: Acta Histochem. 2014 Jan;116(1) Orexin 1 receptor is expressed in the seminiferous tubules of boar testis. PMID: 23746541
  2. Immunoreactivity (IR) for both orexins A and B (OXA and OXB), and cognate receptors (OX1R and OX2R) were identified only in the excretory striated ducts of the MSG while acinar cells were not immunoreactive. PMID: 21888930
  3. Data demonstrated the presence of OX1R and OX2R genes and proteins in the ovary of the pig and the impact of the hormonal milieu on the expression of both receptors. PMID: 20688107
  4. expression of OX1R and Ox2R in hypothalamus during oestrous cycle; evidence OX1R and OX2R mRNAs and proteins occur in hypothalamic structures engaged in control of reproduction; indicate dependence of OXR expression on endocrine reproductive state PMID: 20610868
  5. findings provide the first evidence that orexin receptors 1 and 2 messenger RNAs and proteins occur in the pituitary of the pig and indicate the dependence of orexin receptor expression on the endocrine reproductive state PMID: 19394166

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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