Recombinant Protein let-756 (let-756)

Code CSB-YP612114CXY
MSDS
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Source Yeast
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Code CSB-EP612114CXY
MSDS
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Source E.coli
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Code CSB-EP612114CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP612114CXY
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP612114CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
let-756
Uniprot No.
Alternative Names
let-756; C05D11.4Protein let-756; Lethal protein 756
Species
Caenorhabditis elegans
Expression Region
1-425
Target Protein Sequence
MAVPAASSIV SYGGAATSNF LTTPVTPFLA GFYNSNFVTD RINSCAPYRV DRIRKQLQDE EENGYPPADD RRRGALFCRS GTWLEMLPIE NPDDGSTRVK VHGTKEESSK FSIVEFVSVA MSLVSIRGVE TKNFICMDPS GKLYATPSSN YSTECVFLEE MMENYYNLYA SCAYGDRFNP WYIELRRSGK PRRGPNSKKR RKASHFLVVH HDLDRLRSPV PNGNDVTDLV VASLFHQPPS HPLFRQQTVT KPPNPHRISN LRAKVEMTNQ AEKQRLLEEK KRRREKKKRR REDRLRKEEQ IREARRQELK SLREEELRRR YQQQQQQQAS TQTRYNRPQN PANPYPTYRP LPTRSTVQSP RPAYNPYWQS PVTQAPHHNS HHHHHHHPRV SSSSDPQQRH QSQQHYLAQT VSNPNRQNVN YQRYP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for larval development. Probably by binding receptor egl-15, regulates negatively membrane protrusion from body wall muscles during larval development.
Gene References into Functions
  1. the EFISIA motif in the core of LET-756 is essential for its biological activity PMID: 15199049
  2. LET-756, like vertebrate FGF9, -16, and -20, belongs to the FGF9 family, whereas EGL-17, like vertebrate FGF8, -17, and -18, could be included in the FGF8 family [review] PMID: 15614779
  3. LET-756 exerts its functions through a balance between a synergy of several nuclear localization signals and attenuated secretion signal. PMID: 16487967
Subcellular Location
Nucleus. Membrane; Peripheral membrane protein.
Protein Families
Heparin-binding growth factors family
Tissue Specificity
Expressed in pharynx, CAN neuron and body wall muscles.
Database Links

KEGG: cel:CELE_C05D11.4

STRING: 6239.C05D11.4

UniGene: Cel.19648

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