Recombinant Rat CDK5 regulatory subunit-associated protein 1 (Cdk5rap1)

Code CSB-YP862409RA
MSDS
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Source Yeast
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Code CSB-EP862409RA
MSDS
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Source E.coli
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Code CSB-EP862409RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862409RA
MSDS
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Source Baculovirus
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Code CSB-MP862409RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cdk5rap1
Uniprot No.
Alternative Names
Cdk5rap1CDK5 regulatory subunit-associated protein 1; CDK5 activator-binding protein C42
Species
Rattus norvegicus (Rat)
Expression Region
1-586
Target Protein Sequence
MHPLQRVFRA QRLSAPLTSM CWVLLRTFRA HNSTSCPDPE GKSSEGVQKD FSSRLATGPT FQHFLRSASV PQEKPSSPEV EDPPPYLSGD ELLGRQRKVY LETYGCQMNV NDTEIAWSIL QKSGYLRTSN LQEADVILLV TCSIREKAEQ TIWNRLHQLK VLKAKRPRSR VPLRIGILGC MAERLKGEIL NREKMVDLLA GPDAYRDLPR LLAVVESGQQ AANVLLSLDE TYADIMPVQT SPSATSAFVS IMRGCDNMCS YCIVPFTRGR ERSRPVASIL DEVRKLSEQG LKEVTLLGQN VNSFRDNSEV QFSSTGSANL SRGFTTNYKP KQGGLRFSHL LDQVSRIDPE MRIRFTSPHP KDFPDEVLQL IRERHNICKQ IHLPAQSGSS RVLEAMRRGY SREAYVALVH HIREAIPGVG LSSDFITGFC GETEDDHLQT VSLLREVQYN TGFLFAYSMR QKTRAYHRLK DDVPEEVKLR RLEELITVFR EEASKVNATS VGCTQLVLVE GFSKRSTTDL CGRNDANLKV IFPDAEVEDI TDPGLKVRAQ PGDYVLVKII SASSQTLKGH ILCRTTMKDS SMNCLT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i(6)A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp). Essential for efficient and highly accurate protein translation by the ribosome. Specifically inhibits CDK5 activation by CDK5R1. Essential for efficient mitochondrial protein synthesis and respiratory chain.
Subcellular Location
Mitochondrion inner membrane.
Protein Families
Methylthiotransferase family, MiaB subfamily
Tissue Specificity
Expressed in brain.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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