Recombinant Rat PRKC apoptosis WT1 regulator protein (Pawr)

Code CSB-YP723468RA
MSDS
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Source Yeast
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Code CSB-EP723468RA
MSDS
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Source E.coli
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Code CSB-EP723468RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP723468RA
MSDS
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Source Baculovirus
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Code CSB-MP723468RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Pawr; Par4; PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Par-4; Transcriptional repressor Par-4-like protein PAWR
Species
Rattus norvegicus (Rat)
Expression Region
1-332
Target Protein Sequence
MATGGYRSSG STTDFLEEWK AKREKMRAKQ NPVGPGSSGG DPAAKSPAGP LAQTTAAGTS ELNHGPAGAA APAAPGPGAL NCAHGSSALP RGAPGSRRPE DECPIAAGAA GAPASRGDEE EPDSAPEKGR SSGPSARKGK GQIEKRKLRE KRRSTGVVNI PAAECLDEYE DDEAGQKERK REDAITQQNT IQNEAASLPD PGTSYLPQDP SRTVPGRYKS TISAPEEEIL NRYPRTDRSG FSRHNRDTSA PANFASSSTL EKRIEDLEKE VLRERQENLR LTRLMQDKEE MIGKLKEEID LLNRDLDDME DENEQLKQEN KTLLKVVGQL TR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
Gene References into Functions
  1. this study shows that downregulation of iNOS, IL-1beta, and P2X7 expression in mast cells via activation of PAR4 contributes to the Inhibition of visceral hyperalgesia PMID: 29854833
  2. Phosphorylation by CK2 impairs Par-4 proapoptotic functions. PMID: 24457960
  3. Par-4 binding does not block MYPT1 phosphorylation in rat caudal arterial smooth muscle. PMID: 23219599
  4. Par-4 and caspase-3 immunoreactivity in specific brain areas including the hippocampus CA1, CA3 and dentate gyrus, the adjacent cortex and the striatum in adult and aged rats from control and neonatally ferrous iron (Fe+2)-treated groups. PMID: 20369315
  5. PAR4 activation in the knee joint leads to mast cell-dependent changes in joint mechanosensitivity and pain behavior. PMID: 21238854
  6. depression-like behaviors is associated with down-regulated Par-4 and dopamine receptor D2 gene expression in the striatum through a non-methylation mechanism PMID: 21596067
  7. Par-4 is a new activator of myosin phosphatase PMID: 20130087
  8. Unlike Par-1, Par-2, and Par-3, Par-4 mRNA levels are already markedly upregulated 6 hr after optic nerve crush, reaching a peak of 6.1-fold increase at 12 hr, then declining to just above basal levels by 21 days. PMID: 12836167
  9. Par-4 exhibits an antitransforming capacity by antagonizing p185(BCR-ABL)-induced factor-independent proliferation in hematopoietic cells. PMID: 15246161
  10. Par-4 recruits Dlk to stress fibers, leading to enhanced phosphorylation of the regulatory light chain of myosin II (MLC) and to the induction of apoptosis. PMID: 15817164
  11. the mechanism for Ras-mediated down-regulation of Par-4 is by promoter methylation PMID: 15831492
  12. Amida translocates to the cytoplasm and is recruited to actin filaments by Par-4, resulting in enhanced induction of apoptosis. PMID: 16229834
  13. These findings indicate that FSH, by suppressing Par-4 expression in the ovary, activates PKCzeta-dependent antiapoptotic pathway and suggest that Par-4 is part of the mechanism underlying granulosa cells apoptotic demise. PMID: 17219052
  14. The PAR-4 agonist peptide induces transient [Ca2+]i changes with different kinetics compared to that due to activation of the PAR-1 receptor. This indicates that RBCE cells grown on Matrigel express PAR-4 receptors. PMID: 18294734
  15. Par-4 is a novel regulator of myosin light chain phosphorylation in differentiated, contractile vascular smooth muscle. PMID: 18505470
  16. Under physiological conditions, Par-4 self-associates via the C-terminal domain, forming a coiled-coil. PMID: 19490121
  17. Dlk-mediated phosphorylation of Par-4 at T155 is a crucial event in Dlk/Par-4-induced apoptosis. PMID: 19625447

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Subcellular Location
Cytoplasm. Nucleus.
Database Links
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