Recombinant Rous sarcoma virus Gag-Pro polyprotein (gag-pro)

Code CSB-YP365907RHO
MSDS
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Source Yeast
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Code CSB-EP365907RHO
MSDS
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Source E.coli
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Code CSB-EP365907RHO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP365907RHO
MSDS
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Source Baculovirus
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Code CSB-MP365907RHO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
gagGag polyprotein [Cleaved into: Matrix protein p19; p2A; p2B; p10; Capsid protein p27; alternate cleaved 1; Capsid protein p27; alternate cleaved 2; Spacer peptide; SP; p3); Nucleocapsid protein p12; Protease p15; EC 3.4.23.-)]
Species
Rous sarcoma virus (strain Prague C) (RSV-PrC)
Expression Region
1-155
Target Protein Sequence
MEAVIKVISS ACKTYCGKTS PSKKEIGAML SLLQKEGLLM SPSDLYSPGS WDPITAALSQ RAMILGKSGE LKTWGLVLGA LKAAREEQVT SEQAKFWLGL GGGRVSPPGP ECIEKPATER RIDKGEEVGE TTVQRDAKMA PEETATPKTV GTSCY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The p10 domain folds back and interacts with the capsid protein domain during Gag polyprotein assembly in the immature particle (before the maturation cleavage that splits the 2 domains).; Capsid protein p27: Self-associates to form the irregular polyhedron core composed of hexamers and pentamers, that encapsulates the genomic RNA-nucleocapsid complex. Assembles as a tube in vitro.; Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.; Plays a role in the oligomerization of the Gag polyprotein and in the stabilization of the immature particle. Essential layering element during tube assembly.; Aspartyl protease that mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.
Gene References into Functions
  1. Taken together, the authors interpret these results to mean that Gag membrane interaction is cooperative in that it depends on the ability of Gag to multimerize. PMID: 26676779
  2. The resulting model of the RSV Gag lattice shows features and dynamics of the capsid protein with implications for the maturation process, and confirms the stabilizing role of the upstream and downstream regions of Gag, namely p10 and SP-NC. PMID: 26118533
  3. The structure of immature virus-like Rous sarcoma virus Gag particles reveals a structural role for the p10 domain in assembly. PMID: 26223638
  4. RSV Gag is highly flexible and can adopt a folded-over conformation on a lipid bilayer, implicating both the N and C termini in membrane binding. PMID: 26246573
  5. Taken together, these results provide strong evidence for the formation of a critical six-helix bundle in Gag assembly. PMID: 24599998
  6. These data suggest a possible connection between Gag nuclear trafficking and phosphoinositide-dependent plasma membrane targeting. PMID: 23325682
  7. Taken together, the authors interpret these results to mean that Rous sarcoma virus Gag membrane association is driven by electrostatic interactions and not by any specific association with phosphatidylinositol-(4,5)-bisphosphate. PMID: 21813603
  8. Directionality of nucleocytoplasmic transport of the retroviral gag protein depends on sequential binding of karyopherins and viral RNA. PMID: 20435918
  9. The authors conclude that the wild-type capsid protein sequence represents an evolutionary compromise between competing requirements for optimization of Gag assembly (of the immature virion) and capsid assembly (in the maturing virion). PMID: 20427531
  10. These data support a model in which the gag protein LYPSL motif of Rous sarcoma virus mediates budding through an interaction with the ESCRT adaptor protein Alix. PMID: 20392845
  11. ubiquitination of Gag is dependent upon Nedd4 binding to the L domain during RSV budding pathway PMID: 15564502
  12. the nucleocytoplasmic dynamics of RSV Gag depend upon the counterbalance of Crm1p-mediated export with two independent NLSs, each interacting with distinct nuclear import factors. PMID: 16439536
  13. These results indicate that the p10 NES domain of Gag is critical for virus replication and that it plays overlapping roles required for the nuclear shuttling of Gag and for the maintenance of proper virion core morphology. PMID: 17634229
  14. these experimental results strongly suggest that Rous sarcoma virus Gag proteins are capable of interacting within the nucleus. PMID: 17977961
  15. These findings provide important biological evidence in support of a significant conformational change involving the dimerization helix and the major homology region during capsid protein maturation. PMID: 18433823
  16. Taken together, these results define a short assembly domain between the folded portions of CA and NC, which is essential for formation of the immature Gag shell. PMID: 18448521
  17. cryo-electron microscopic analyses of two in-vitro-assembled capsids of Rous sarcoma virus PMID: 19194444
  18. Inserting a bipartite nuclear localization signal into Gag enhanced nuclear localization and restored RNA packaging in mutant viruses. PMID: 19369339

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Subcellular Location
[Matrix protein p19]: Virion.; [Capsid protein p27, alternate cleaved 1]: Virion.; [Capsid protein p27, alternate cleaved 2]: Virion.; [Nucleocapsid protein p12]: Virion.; [Gag polyprotein]: Host nucleus, host nucleolus. Host nucleus, host nucleoplasm.
Database Links

KEGG: vg:1491923

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