Recombinant Saccharomyces cerevisiae DNA topoisomerase 2 (TOP2), partial

Code CSB-YP361947SVG
MSDS
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Source Yeast
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Code CSB-EP361947SVG
MSDS
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Source E.coli
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Code CSB-EP361947SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP361947SVG
MSDS
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Source Baculovirus
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Code CSB-MP361947SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
TOP2; TOR3; YNL088W; N2244; DNA topoisomerase 2; EC 5.6.2.2; DNA topoisomerase II
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.
Gene References into Functions
  1. Condensin DNA supercoiling activity correlates with Top2 intertwine removal during DNA replication. PMID: 27716481
  2. Topoisomerase II plays a central role in a molecular pathway for crossover interference PMID: 25043020
  3. Chromatin regulates DNA torsional energy via topoisomerase II-mediated relaxation of positive supercoils. PMID: 24859967
  4. the cell cycle delay depends on the divergent and catalytically inert C-terminal region of Topo II, indicating that transmission of a checkpoint signal may occur via the C-terminus PMID: 24098144
  5. Structure of topoisomerase II-DNA-nucleotide complex produces an unexpected interaction between DNA and a conformational transducing element in the ATPase domain, which is critical for both DNA-stimulated ATP hydrolysis and global topoisomerase activity. PMID: 23022727
  6. a topological change on DNA drives topoisomerase II to decatenate molecules during mitosis, potentially driving the full decatenation of the genome PMID: 21393545
  7. In eukaryotes, replication fork barriers, Rrm3, and Top2 coordinate replication fork progression and fusion at chromosomal termination regions, thus counteracting abnormal genomic transitions. PMID: 20797631
  8. structure of the DNA-binding and cleavage core of Saccharomyces cerevisiae topoisomerase II covalently linked to DNA through its active-site tyrosine at 2.5A resolution PMID: 20485342
  9. compromised Topo II function activates a yeast checkpoint system that operates by a novel mechanism PMID: 16651657
  10. structure of a complex between the DNA-binding and cleavage core of Saccharomyces cerevisiae Topo II (also known as Top2) and a gate-DNA segment PMID: 18097402
  11. kinetic and structural analysis of coupling between ATP binding and DNA cleavage by DNA topoisomerase II PMID: 18403371
  12. Top2 seems to play a novel role in centromeric chromatin compaction that is distinct from decatenation PMID: 18701701
  13. analysis of Top2 covalent complexes containing DNA single strand breaks PMID: 18723844

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Subcellular Location
Nucleus.
Protein Families
Type II topoisomerase family
Database Links

KEGG: sce:YNL088W

STRING: 4932.YNL088W

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