Recombinant Saccharomyces cerevisiae Non-histone chromosomal protein 6A (NHP6A)

Code CSB-YP320019SVG
MSDS
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Source Yeast
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Code CSB-EP320019SVG
MSDS
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Source E.coli
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Code CSB-EP320019SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP320019SVG
MSDS
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Source Baculovirus
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Code CSB-MP320019SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NHP6A
Uniprot No.
Alternative Names
NHP6A; NHPA; YPR052C; YP9499.09C; Non-histone chromosomal protein 6A
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-93
Target Protein Sequence
MVTPREPKKR TTRKKKDPNA PKRALSAYMF FANENRDIVR SENPDITFGQ VGKKLGEKWK ALTPEEKQPY EAKAQADKKR YESEKELYNA TLA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6B.
Gene References into Functions
  1. The yeast HMG box protein NHP6A shows DNA-segment-facilitated dissociation effects similar to those of Fis. PMID: 26220077
  2. adapt two high-resolution in vivo protein-mapping techniques to demonstrate direct binding of the heterologous Nhp6A protein at a LacI repression loop in living E. coli cells PMID: 26039992
  3. Observations support a model in which the HMGB protein Nhp6A soften DNA through random dynamic binding and dissociation, accompanied by DNA kinking and straightening, respectively. PMID: 23221634
  4. While all of the HMGB proteins bend DNA to preferred angles, Nhp6A promoted the formation of higher-order oligomer structures. PMID: 22197373
  5. Chromatin-dependent binding of the S. cerevisiae HMGB protein Nhp6A affects nucleosome dynamics and transcription PMID: 20844014
  6. Analysis of mechanical response generated by binding of DNA-bending protein NHP6A to single tethered 48.5 kb lambda-DNA molecules finds that compaction of DNA increases with increasing NHP6A concentration. PMID: 15504049
  7. NHP6A is required for transcription of a subset of tRNA genes and heterochromatin barrier function. PMID: 17178828
  8. Nhp6Ap has a role in the evolutionarily conserved calmodulin-dependent nuclear import pathway PMID: 17878171
  9. An isolated HMG box A domain from human HMGB2 does not enhance DNA flexibility. Substitution of a small number of cationic residues from the N-terminal leader of a functional yeast box B protein, Nhp6Ap, confers the ability to enhance DNA flexibility. PMID: 19236006

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Subcellular Location
Nucleus. Chromosome. Note=Colocalizes with both RNA polymerase II and some regions that are not transcribed on chromatin.
Protein Families
NHP6 family
Database Links

KEGG: sce:YPR052C

STRING: 4932.YPR052C

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