This recombinant Saccharomyces cerevisiae Transketolase 1 (TKL1) is a semi-custom product. There are 5 expression system options: Yeast, E. coli, In Vivo Biotinylation in E. coli, Baculovirus, and Mammalian cell. Your requirements will be given top priority in determining the protein tags. For proteins within 800 aa, risk-free custom service is guaranteed. It means you will not be charged if the protein cannot be delivered.
Saccharomyces cerevisiae TKL1 is an enzyme crucial for the pentose phosphate pathway (PPP). TKL1 transfers a ketol group from xylulose-5-phosphate to ribose-5-phosphate, generating glyceraldehyde-3-phosphate and sedoheptulose-7-phosphate [1]. The PPP is important for nucleotide and lipid biosynthesis and maintaining cellular redox balance [2]. Overexpression of TKL1 and other PPP enzymes like transaldolase increased the flux through the pentose phosphate pathway in Saccharomyces cerevisiae [3]. TKL1 is also a key enzyme in the non-oxidative pentose phosphate pathway and is essential for the fermentation rate of xylulose in yeast [4][5].
References:
[1] H. Iwata, Y. Kobayashi, D. Mizushima, T. Watanabe, J. Ogihara, & T. Kasumi, Complementary function of two transketolase isoforms from moniliella megachiliensis in relation to stress response, Amb Express, vol. 7, no. 1, 2017. https://doi.org/10.1186/s13568-017-0342-0
[2] B. Johansson and B. Hahn-Hägerdal, The non-oxidative pentose phosphate pathway controls the fermentation rate of xylulose but not of xylose in tmb3001, Fems Yeast Research, vol. 2, no. 3, p. 277-282, 2002. https://doi.org/10.1016/s1567-1356(02)00114-9
[3] M. Walfridsson, J. Hallborn, M. Penttilä, S. Keränen, & B. Hahn‐Hägerdal, Xylose-metabolizing saccharomyces cerevisiae strains overexpressing the tkl1 and tal1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase, Applied and Environmental Microbiology, vol. 61, no. 12, p. 4184-4190, 1995. https://doi.org/10.1128/aem.61.12.4184-4190.1995
[4] B. Johansson and B. Hahn-Hägerdal, The non-oxidative pentose phosphate pathway controls the fermentation rate of xylulose but not of xylose in tmb3001, Fems Yeast Research, vol. 2, no. 3, p. 277-282, 2002. https://doi.org/10.1016/s1567-1356(02)00114-9
[5] B. Johansson and B. Hahn‐Hägerdal, The non-oxidative pentose phosphate pathway controls the fermentation rate of xylulose but not of xylose insaccharomyces cerevisiaetmb3001, Fems Yeast Research, vol. 2, no. 3, p. 277-282, 2002. https://doi.org/10.1111/j.1567-1364.2002.tb00095.x
