Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
GRA3; Tg556; Dense granule protein 3; P30
Species
Toxoplasma gondii
Expression Region
43-114aa
Target Protein Sequence
ADQPENHQALAEPVTGVGEAGVSPVNEAGESYSSATSGVQEATAPGAVLLDAIDAESDKVDNQAEGGERMKK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 43-114 constitute the expression domain of recombinant Toxoplasma gondii GRA3. The theoretical molecular weight of the GRA3 protein is 10.8 kDa. This GRA3 protein is produced using e.coli expression system. The GRA3 gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant GRA3 protein during the following stages.
Toxoplasma gondii dense granule protein 3 (GRA3) is a protein involved in the early stages of infection and is believed to play a role in the modulation of the host cell environment. It may contribute to the formation and maintenance of the parasitophorous vacuole. GRA3 is recognized by the host immune system, leading to the production of antibodies during T. gondii infection. It is considered a potential target for vaccine development. The immune response against GRA3 is part of the host defense mechanism against T. gondii. Understanding the interaction between GRA3 and the host immune system provides insights into the pathogenesis of toxoplasmosis.
