Recombinant Xenopus tropicalis POU domain, class 5, transcription factor 1.1 (pou5f1.1)

Code CSB-YP460983XBF
MSDS
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Source Yeast
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Code CSB-EP460983XBF
MSDS
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Source E.coli
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Code CSB-EP460983XBF-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP460983XBF
MSDS
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Source Baculovirus
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Code CSB-MP460983XBF
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pou5f1.1
Uniprot No.
Alternative Names
pou5f1.1; oct25; POU domain; class 5; transcription factor 1.1; POU class V protein oct-25
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region
1-459
Target Protein Sequence
MYSQQPFPPF AFNAGLIQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGD SGVQGMGDCS APVMPWNSMA SFDHHGQVET NQQGNPIRAP SPTPTLSDSR IKVKEEVAHE TDSGEESPEP KYPSPPNPSL YYPNTWTGSP FWQVNPTAGN NSNSTNPMPS QTLVKNGSLP GNTTYPTPAN QSPNTPVDCV VSSMESSRCS SANSSNGAIN ERATTIPNGG MVDGGQSSDN EEEVPSESEM EQFAKDLKHK RVSMGYTQAD VGYALGVLYG KMFSQTTICR FESLQLSFKN MCQLKPFLER WLVEAENNDN LQELINREQV IAQTRKRKRR TNIENIVKGT LESYFMKCSK PGAQEMVQIA KELNMDKDVV RVWFCNRRQK GKRQGMPTVD ENDGEGYDVG QTMASPPVGH YSLPQVVTSQ GYMAAPLGST PPLYASAFHK NELFPQPLPH AMPMGGHIG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription.
Subcellular Location
Nucleus.
Protein Families
POU transcription factor family, Class-5 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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