Code | CSB-RA004972A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:500 |
IF | 1:30-1:200 |
The CD97 recombinant monoclonal antibody is generated through a combination of protein technology and DNA recombinant techniques. It begins with the immunization of animals using a synthetic peptide derived from human CD97, resulting in the production of B cells. Positive B cells are then isolated and subjected to single clone identification. The genes encoding the light and heavy chains of the CD97 antibody are amplified using PCR and inserted into a plasmid vector to create a recombinant vector, which is introduced into host cells for antibody expression. The CD97 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. Stringent validation procedures ensure its accuracy and suitability for ELISA, IHC, and IF applications. With its ability to specifically detect human CD97 protein, the CD97 recombinant monoclonal antibody is a valuable tool for research purposes.
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