Code | CSB-RA155442A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The production of the FADS1 recombinant monoclonal antibody involves a well-defined process to ensure its efficacy and specificity. Initially, B cells are isolated from an immunized animal using a synthesized peptide derived from human FADS1 as the immunogen. Subsequently, total RNA is extracted from the isolated B cells and converted into cDNA through reverse transcription. The FADS1 antibody genes are amplified using PCR with primers specific to the antibody constant regions and inserted into an expression vector. This vector is then introduced into host cells, enabling the production of the FADS1 recombinant monoclonal antibody. The antibody is harvested from the cell culture supernatant and purified using affinity chromatography to obtain a highly purified and concentrated preparation. Extensive characterization assays, including ELISA, WB, IHC, IF, and FC analysis, are performed to validate the specificity and functionality of the antibody, confirming its ability to bind specifically to human FADS1 protein.
There are currently no reviews for this product.