Code | CSB-RA261933A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The IKBKB recombinant monoclonal antibody is developed using protein and DNA recombinant technology. Initially, a synthesized peptide derived from human IKBKB is used to immunize mice. After some time, the mice's spleen is removed under sterile conditions, and the cDNA synthesized from the RNA reverse transcription of the spleen cells' total RNA is used as a template to amplify the IKBKB antibody gene through PCR. Subsequently, the IKBKB antibody gene is inserted into a vector, which is then transfected into host cells for further culturing. The IKBKB recombinant monoclonal antibody is purified from the host cells' supernatant via affinity chromatography and subjected to rigorous testing. It can be used for detecting human IKBKB protein in ELISA and WB experimental techniques.
The IKBKB protein, also known as IKKβ, is a protein kinase that plays a key role in the regulation of the NFκB signaling pathway. This pathway is involved in the regulation of immune responses, inflammation, and cell survival. Dysregulation of the NFκB pathway has been implicated in various diseases, including cancer and autoimmune disorders. In addition to its role in the NFκB pathway, IKBKB also has other functions in cells, such as regulation of the cell cycle and modulation of the Wnt signaling pathway.
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