Code | CSB-RA256500A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
CUSABIO generated IKBKB antibody-producing hybridomas by fusing myeloma cells to B cells extracted from the animal immunized with a synthetic peptide derived from human IKBKB. The variable light and variable heavy domains of IKBKB antibody-producing hybridomas were sequenced, and the gene was inserted into a vector. Subsequently, the IKBKB monoclonal antibody gene-containing vector was transfected into cells for cultivation, and the IKBKB recombinant monoclonal antibody was purified using affinity chromatography from the cell culture supernatant. The purified antibody was specifically tested for human IKBKB protein detection in ELISA, WB, IHC, and IF applications.
The IKBKB protein, also known as IKKβ, is a serine/threonine protein kinase that plays a critical role in the regulation of the NFκB signaling pathway. It is involved in the regulation of various cellular processes, including immune response, inflammation, cell growth, and differentiation. Specifically, IKBKB is responsible for the phosphorylation and subsequent degradation of the inhibitor protein IκB, leading to the release and nuclear translocation of NFκB. Once in the nucleus, NFκB activates the transcription of genes involved in inflammatory and immune responses, cell survival, and proliferation, among other functions.
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