Code | CSB-RA013481A396phHU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
In the quest to develop the phospho-MAPT (S396) recombinant monoclonal antibody, the initial phase involves the extraction of genes encoding this antibody from rabbits that have been previously exposed to a synthesized peptide derived from the human MAPT protein phosphorylated at S396. These antibody genes are then seamlessly integrated into specialized expression vectors. Following this genetic modification, the vectors are carefully introduced into host suspension cells, which are diligently cultured to stimulate the expression and secretion of antibodies. Subsequently, the phospho-MAPT (S396) recombinant monoclonal antibody is subjected to a meticulous purification process utilizing affinity chromatography, effectively isolating the antibody from the surrounding cell culture supernatant. Finally, the antibody's functionality is comprehensively evaluated through ELISA and WB tests, unequivocally confirming its capacity to interact effectively with the human MAPT protein phosphorylated at S396.
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