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Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA017408A474phHU
Size US$210
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  • Western Blot
    Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not)
    All lanes Phospho-PAK4/PAK5/PAK6 antibody at 2.15μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 64,75,80 KDa
    Observed band size: 75,80 KDa
  • Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA017408A474phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Product Details

Uniprot No.
O96013 Q9P286 Q9NQU5
Target Names
PAK4/PAK5/PAK6
Species Reactivity
Human
Immunogen
A synthesized peptide derived from Human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
2D1
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:20-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To make the phospho-PAK4/PAK5/PAK6 (S474/S560/S602) recombinant monoclonal antibody, the first step is to isolate the genes coding for the phospho-PAK4/PAK5/PAK6 (S474/S560/S602) antibody from the rabbits immunized with a synthesized peptide derived from human phospho-PAK4/PAK5/PAK6 (S474/S560/S602) protein. Secondly, these antibody genes are cloned into expression vectors. Thirdly, the modified vectors are transfected into host suspension cells. Fourthly, positive cells are cultured to express and secrete antibodies. The phospho-PAK4/PAK5/PAK6 (S474/S560/S602) recombinant monoclonal antibody is purified from the cell culture supernatant through affinity chromatography. Finally, the activity of the antibody is tested in ELISA, WB, and IF tests. This antibody can react with human phospho-PAK4/PAK5/PAK6 (S474/S560/S602) protein.

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