Code | CSB-RA618984A380phHU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
IP | 1:200-1:1000 |
To manufacture the phospho-RPS6KA1 (S380) recombinant monoclonal antibody, the journey begins with the retrieval of genes responsible for encoding this antibody from rabbits that have previously undergone immunization with a synthesized peptide originating from the human RPS6KA1 protein phosphorylated at S380. Subsequently, these antibody genes are seamlessly integrated into specialized expression vectors. Following this genetic modification, the vectors are thoughtfully introduced into host suspension cells, which are diligently cultivated to encourage the production and secretion of antibodies. After this growth phase, the phospho-RPS6KA1 (S380) recombinant monoclonal antibody undergoes a meticulous purification process using affinity chromatography techniques, effectively isolating the antibody from the surrounding cell culture supernatant. Lastly, the functionality of the antibody is rigorously assessed through a battery of tests, including ELISA, WB, and IP, conclusively affirming its ability to effectively react with the human RPS6KA1 protein phosphorylated at S380.
Phosphorylation of RPS6KA1 at S380 is a crucial regulatory mechanism that allows cells to respond to extracellular signals and stressors, modulating gene expression and influencing various cellular processes, including cell growth and stress responses. Dysregulation of this phosphorylation event can have significant implications in diseases and conditions related to cell proliferation and gene expression.
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