PCSK9, preprotein convertase Bacillus subtilis protease/kexin type 9, is a liver-synthesised and secreted enzyme encoded by the PCSK9 gene.The mechanism of action of PCSK9 mainly involves the regulation of blood levels of low-density lipoprotein receptor (LDLR), which affects blood levels of LDL-cholesterol by binding to the LDLR and promoting its degradation in the liver. PCSK9 plays an important role in the maintenance of lipid homeostasis, and its abnormal function has been associated with diseases such as familial hypercholesterolaemia.The development of PCSK9 inhibitors provides new therapeutic strategies to reduce the risk of cardiovascular diseases.
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
The purity of PCSK9 was greater than 95% as determined by SEC-HPLC
PCSK9 Recombinant Monoclonal Antibody (CSB-RA259644A0HU)
Validated Data
Western Blot
Positive WB detected in: PC-3 whole cell lysate
All lanes: PCSK9 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 75, 21 kDa
Observed band size: 60, 75 kDa
IHC image of CSB-RA259644A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Human Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit (CSB-EL017647HU)
Validated Data
Code: CSB-EL017647HU
Size: 96T,5×96T,10×96T
Sensitivity: 0.11 ng/mL
Detection Range: 0.45 ng/mL-30 ng/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following Pcsk9 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Pcsk9 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA017647GA01HU | PCSK9 Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA638311 | PCSK9 Antibody |
Human | ELISA,WB |
CSB-PA108805 | PCSK9 Antibody |
Human | ELISA,WB |
CSB-PA07039A0Rb | PCSK9 Antibody |
Human | ELISA, IHC, IF |
CSB-PA07039B0Rb | PCSK9 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA07039C0Rb | PCSK9 Antibody, FITC conjugated |
Human | |
CSB-PA07039D0Rb | PCSK9 Antibody, Biotin conjugated |
Human | ELISA |
CSB-PA017647LA01HU | PCSK9 Antibody |
Human | ELISA, WB, IHC, IF |
CSB-PA017647LB01HU | PCSK9 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA017647LC01HU | PCSK9 Antibody, FITC conjugated |
Human | |
CSB-PA017647LD01HU | PCSK9 Antibody, Biotin conjugated |
Human | ELISA |
CSB-RA259644A0HU | PCSK9 Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC |
Pcsk9 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP017647RA CSB-EP017647RA CSB-BP017647RA CSB-MP017647RA CSB-EP017647RA-B |
Recombinant Rat Proprotein convertase subtilisin/kexin type 9 (Pcsk9) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Macaca mulatta (Rhesus macaque)
Code | Product Name | Source |
---|---|---|
CSB-YP017647MOW CSB-EP017647MOW CSB-BP017647MOW CSB-MP017647MOW CSB-EP017647MOW-B |
Recombinant Macaca mulatta (Rhesus macaque) Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Colobus guereza (Mantled guereza) (Eastern black-and-white colobus monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP017647DVH CSB-EP017647DVH CSB-BP017647DVH CSB-MP017647DVH CSB-EP017647DVH-B |
Recombinant Colobus guereza Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Lagothrix lagotricha (Brown woolly monkey) (Humboldt's woolly monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP017647LNL CSB-EP017647LNL CSB-BP017647LNL CSB-MP017647LNL CSB-EP017647LNL-B |
Recombinant Lagothrix lagotricha Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Ateles geoffroyi (Black-handed spider monkey) (Geoffroy's spider monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP017647AVF CSB-EP017647AVF CSB-BP017647AVF CSB-MP017647AVF CSB-EP017647AVF-B |
Recombinant Ateles geoffroyi Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Pan paniscus (Pygmy chimpanzee) (Bonobo)
Code | Product Name | Source |
---|---|---|
CSB-YP017647EQU CSB-EP017647EQU CSB-BP017647EQU CSB-MP017647EQU CSB-EP017647EQU-B |
Recombinant Pan paniscus Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Pongo pygmaeus (Bornean orangutan)
Code | Product Name | Source |
---|---|---|
CSB-YP017647EXP CSB-EP017647EXP CSB-BP017647EXP CSB-MP017647EXP CSB-EP017647EXP-B |
Recombinant Pongo pygmaeus Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Saguinus labiatus (Red-chested mustached tamarin)
Code | Product Name | Source |
---|---|---|
CSB-YP017647SVY CSB-EP017647SVY CSB-BP017647SVY CSB-MP017647SVY CSB-EP017647SVY-B |
Recombinant Saguinus labiatus Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Pan troglodytes (Chimpanzee)
Code | Product Name | Source |
---|---|---|
CSB-YP017647EQV CSB-EP017647EQV CSB-BP017647EQV CSB-MP017647EQV CSB-EP017647EQV-B |
Recombinant Pan troglodytes Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Plecturocebus moloch (Dusky titi monkey) (Callicebus moloch)
Code | Product Name | Source |
---|---|---|
CSB-YP017647CYE CSB-EP017647CYE CSB-BP017647CYE CSB-MP017647CYE CSB-EP017647CYE-B |
Recombinant Callicebus moloch Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Gorilla gorilla gorilla (Western lowland gorilla)
Code | Product Name | Source |
---|---|---|
CSB-YP017647GGZ CSB-EP017647GGZ CSB-BP017647GGZ CSB-MP017647GGZ CSB-EP017647GGZ-B |
Recombinant Gorilla gorilla gorilla Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Callithrix jacchus (White-tufted-ear marmoset)
Code | Product Name | Source |
---|---|---|
CSB-YP017647CYL CSB-EP017647CYL CSB-BP017647CYL CSB-MP017647CYL CSB-EP017647CYL-B |
Recombinant Callithrix jacchus Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Macaca nemestrina (Pig-tailed macaque)
Code | Product Name | Source |
---|---|---|
CSB-YP017647MOX CSB-EP017647MOX CSB-BP017647MOX CSB-MP017647MOX CSB-EP017647MOX-B |
Recombinant Macaca nemestrina Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Saimiri boliviensis boliviensis (Bolivian squirrel monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP017647SWC CSB-EP017647SWC CSB-BP017647SWC CSB-MP017647SWC CSB-EP017647SWC-B |
Recombinant Saimiri boliviensis boliviensis Proprotein convertase subtilisin/kexin type 9 (PCSK9), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP017647MO CSB-EP017647MO CSB-BP017647MO CSB-MP017647MO CSB-EP017647MO-B |
Recombinant Mouse Proprotein convertase subtilisin/kexin type 9 (Pcsk9) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Pcsk9 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP017647HU CSB-EP017647HU CSB-BP017647HU CSB-MP017647HU CSB-EP017647HU-B |
Recombinant Human Proprotein convertase subtilisin/kexin type 9 (PCSK9) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-AP005741HU | Recombinant Human Proprotein convertase subtilisin/kexin type 9 (PCSK9) (Active) |
Mammalian cell |
CSB-MP017647HU(M) | Recombinant Human Proprotein convertase subtilisin/kexin type 9 (PCSK9) (D374Y), partial |
Mammalian cell |
CSB-MP017647HU(M1) | Recombinant Human Proprotein convertase subtilisin/kexin type 9 (PCSK9) (R194A), partial |
Mammalian cell |
CSB-MP017647HU(M)-B | Recombinant Human Proprotein convertase subtilisin/kexin type 9 (PCSK9)(D374Y), partial,Biotinylated |
Mammalian cell |
Pcsk9 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL017647HU | Human Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit |
serum, plasma, tissue homogenates | 0.11 ng/mL |
Pcsk9 ELISA Kit for Monkey
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL017647RH | Monkey Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit |
serum, plasma, tissue homogenates | 1.1 pg/mL |
Pcsk9 ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL017647RA | Rat Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit |
serum, plasma, tissue homogenates | 46.88 pg/mL |